3IM4

Crystal structure of cAMP-dependent Protein Kinase A Regulatory Subunit I alpha in complex with dual-specific A-Kinase Anchoring Protein 2

Summary for 3IM4

• Entry DOI: 10.2210/pdb3im4/pdb
• Related: 3IM3
• Descriptor: cAMP-dependent protein kinase type I-alpha regulatory subunit, Dual specificity A kinase-anchoring protein 2, ZINC ION, ... (4 entities in total)
• Functional Keywords: four-helix bundle, acetylation, camp, camp-binding, disulfide bond, nucleotide-binding, phosphoprotein, cytoplasm, membrane, mitochondrion, polymorphism, transit peptide, structural protein, signaling protein
• Biological source: Bos taurus (bovine); More
• Cellular location: Mitochondrion: O43572
• Total number of polymer chains: 3
• Total formula weight: 17425.66

Authors

Sarma, G.N.,Kinderman, F.S.,Kim, C.,von Daake, S.,Taylor, S.S. (deposition date: 2009-08-09, release date: 2010-02-02, Last modification date: 2024-11-27)

Primary citation

Sarma, G.N.,Kinderman, F.S.,Kim, C.,von Daake, S.,Chen, L.,Wang, B.C.,Taylor, S.S.
Structure of D-AKAP2:PKA RI Complex: Insights into AKAP Specificity and Selectivity
Structure, 18:155-166, 2010

PubMed Abstract: A-kinase anchoring proteins (AKAPs) regulate cyclic AMP-dependent protein kinase (PKA) signaling in space and time. Dual-specific AKAP 2 (D-AKAP2) binds to the dimerization/docking (D/D) domain of both RI and RII regulatory subunits of PKA with high affinity. Here we have determined the structures of the RIalpha D/D domain alone and in complex with D-AKAP2. The D/D domain presents an extensive surface for binding through a well-formed N-terminal helix, and this surface restricts the diversity of AKAPs that can interact. The structures also underscore the importance of a redox-sensitive disulfide in affecting AKAP binding. An unexpected shift in the helical register of D-AKAP2 compared to the RIIalpha:D-AKAP2 complex structure makes the mode of binding to RIalpha novel. Finally, the comparison allows us to deduce a molecular explanation for the sequence and spatial determinants of AKAP specificity.

PubMed: 20159461
DOI: 10.1016/j.str.2009.12.012

Experimental method

X-RAY DIFFRACTION (2.285 Å)