3I6O

Crystal structure of wild type HIV-1 protease with macrocyclic inhibitor GRL-0216A

3I6O の概要

• エントリーDOI: 10.2210/pdb3i6o/pdb
• 関連するPDBエントリー: 2HB3 2IEN 2Z4O 3DJK 3DK1 3H5B
• 分子名称: Protease, SODIUM ION, IODIDE ION, ... (6 entities in total)
• 機能のキーワード: hiv-1, wild type protease, protease inhibitor, macrocyclic ligand, aids, aspartyl protease, hydrolase
• 由来する生物種: Human immunodeficiency virus type 1 (BRU ISOLATE) (HIV-1)
• 細胞内の位置: Gag-Pol polyprotein: Host cell membrane ; Lipid-anchor. Matrix protein p17: Virion membrane; Lipid- anchor . Capsid protein p24: Virion . Nucleocapsid protein p7: Virion . Reverse transcriptase/ribonuclease H: Virion . Integrase: Virion : P03367
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 24753.45

構造登録者

Chumanevich, A.A.,Wang, Y.-F.,Kovalevsky, A.Y.,Weber, I.T. (登録日: 2009-07-07, 公開日: 2009-09-29, 最終更新日: 2023-09-06)

主引用文献

Ghosh, A.K.,Kulkarni, S.,Anderson, D.D.,Hong, L.,Baldridge, A.,Wang, Y.F.,Chumanevich, A.A.,Kovalevsky, A.Y.,Tojo, Y.,Amano, M.,Koh, Y.,Tang, J.,Weber, I.T.,Mitsuya, H.
Design, Synthesis, Protein-Ligand X-ray Structure, and Biological Evaluation of a Series of Novel Macrocyclic Human Immunodeficiency Virus-1 Protease Inhibitors to Combat Drug Resistance.
J.Med.Chem., 52:7689-7705, 2009

PubMed Abstract: The structure-based design, synthesis, and biological evaluation of a series of nonpeptidic macrocyclic HIV protease inhibitors are described. The inhibitors are designed to effectively fill in the hydrophobic pocket in the S1'-S2' subsites and retain all major hydrogen bonding interactions with the protein backbone similar to darunavir (1) or inhibitor 2. The ring size, the effect of methyl substitution, and unsaturation within the macrocyclic ring structure were assessed. In general, cyclic inhibitors were significantly more potent than their acyclic homologues, saturated rings were less active than their unsaturated analogues and a preference for 10- and 13-membered macrocylic rings was revealed. The addition of methyl substituents resulted in a reduction of potency. Both inhibitors 14b and 14c exhibited marked enzyme inhibitory and antiviral activity, and they exerted potent activity against multidrug-resistant HIV-1 variants. Protein-ligand X-ray structures of inhibitors 2 and 14c provided critical molecular insights into the ligand-binding site interactions.

PubMed: 19746963
DOI: 10.1021/jm900695w

実験手法

X-RAY DIFFRACTION (1.17 Å)