3HLN
Crystal structure of ClpP A153C mutant with inter-heptamer disulfide bonds
Summary for 3HLN
| Entry DOI | 10.2210/pdb3hln/pdb |
| Descriptor | ATP-dependent Clp protease proteolytic subunit, CALCIUM ION (2 entities in total) |
| Functional Keywords | disulfide bond, disordered equatorial loops, atp-binding, hydrolase, nucleotide-binding, protease, serine protease, stress response, zymogen |
| Biological source | Escherichia coli |
| Cellular location | Cytoplasm: P0A6G7 |
| Total number of polymer chains | 28 |
| Total formula weight | 605851.00 |
| Authors | Kimber, M.S.,Yu, A.Y.H.,Borg, M.,Chan, H.S.,Houry, W.A. (deposition date: 2009-05-27, release date: 2010-07-28, Last modification date: 2024-11-06) |
| Primary citation | Kimber, M.S.,Yu, A.Y.,Borg, M.,Leung, E.,Chan, H.S.,Houry, W.A. Structural and Theoretical Studies Indicate that the Cylindrical Protease ClpP Samples Extended and Compact Conformations. Structure, 18:798-808, 2010 Cited by PubMed Abstract: The highly conserved ClpP protease consists of two heptameric rings that interact by the interdigitation of an alpha-helix beta strand handle domain motif to form a tetradecameric cylinder. We previously proposed that protease dynamics results in the temporary unstructuring of interacting pairs of handle domains, opening transient equatorial side pores that allow for peptide egress. Here, we report the structure of an Escherichia coli ClpP mutant in which each opposing pair of protomers is linked by a disulfide bond. This structure resembles the compact structures of Streptococcus pneumoniae, Mycobacterium tuberculosis, and Plasmodium falciparum ClpPs, rather than the active, extended structures that have previously been determined for E. coli ClpPs. The structural data, along with normal mode analysis, support a model whereby the ClpP cylinder switches dynamically between an active extended state required for substrate degradation and an inactive compact state allowing peptide product release. PubMed: 20637416DOI: 10.1016/j.str.2010.04.008 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (3.2 Å) |
Structure validation
Download full validation report
