3HLN
Crystal structure of ClpP A153C mutant with inter-heptamer disulfide bonds
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-03-18 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0332 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 182.299, 182.299, 476.859 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.220 - 3.200 |
R-factor | 0.21542 |
Rwork | 0.213 |
R-free | 0.25274 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2fzs |
RMSD bond length | 0.007 |
RMSD bond angle | 0.987 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.220 | 3.310 |
High resolution limit [Å] | 3.200 | 3.200 |
Number of reflections | 148306 | |
<I/σ(I)> | 14.2 | 2.1 |
Completeness [%] | 97.5 | 91.7 |
Redundancy | 3 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 1 M 1,6-hexanediol, 0.1 M Sodium acetate pH 4.6, 10 mM CoCl2, 100 mM CaCl2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |