3HIV

Crystal structure of Saporin-L1 in complex with the trinucleotide inhibitor, a transition state analogue

Summary for 3HIV

• Entry DOI: 10.2210/pdb3hiv/pdb
• Related: 3HIO 3HIQ 3HIS 3HIT 3HIW
• Descriptor: Vacuolar saporin, (2R,3R,4R,5R)-5-(2-amino-6-oxo-3,6-dihydro-9H-purin-9-yl)-2-({[(S)-({(3R,4R)-4-({[(S)-{[(2R,3R,4R,5R)-5-(2-amino-6-oxo-6,8-dihydro-9H-purin-9-yl)-2-(hydroxymethyl)-4-methoxytetrahydrofuran-3-yl]oxy}(hydroxy)phosphoryl]oxy}methyl)-1-[(4-amino-5H-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]pyrrolidin-3-yl}oxy)(hydroxy)phosphoryl]oxy}methyl)-4-methoxytetrahydrofuran-3-yl 3-hydroxypropyl hydrogen (S)-phosphate (3 entities in total)
• Functional Keywords: transition state, ribosome inactivating proteins, rips, hydrolase, plant defense, protein synthesis inhibitor, toxin, hydrolase-hydrolase inhibitor complex, hydrolase/hydrolase inhibitor
• Biological source: Saponaria officinalis (Common soapwort)
• Total number of polymer chains: 2
• Total formula weight: 59775.18

Authors

Ho, M.,Sturm, M.B.,Almo, S.C.,Schramm, V.L. (deposition date: 2009-05-20, release date: 2009-12-08, Last modification date: 2024-02-21)

• Primary citation: Ho, M.C.,Sturm, M.B.,Almo, S.C.,Schramm, V.L.; Transition state analogues in structures of ricin and saporin ribosome-inactivating proteins.; Proc.Natl.Acad.Sci.USA, 106:20276-20281, 2009; PubMed: 19920175; DOI: 10.1073/pnas.0911606106

Experimental method

X-RAY DIFFRACTION (2.14 Å)