3GZN

Structure of NEDD8-activating enzyme in complex with NEDD8 and MLN4924

Summary for 3GZN

• Entry DOI: 10.2210/pdb3gzn/pdb
• Descriptor: NEDD8-activating enzyme E1 regulatory subunit, NEDD8-activating enzyme E1 catalytic subunit, NEDD8, ... (5 entities in total)
• Functional Keywords: nedd8, e1-activating enzyme, mln4924, protein binding-ligase complex, protein binding/ligase
• Biological source: Homo sapiens (human); More
• Cellular location: Cell membrane: Q13564; Nucleus: Q15843
• Total number of polymer chains: 6
• Total formula weight: 244266.13

Authors

Sintchak, M.D. (deposition date: 2009-04-07, release date: 2010-02-02, Last modification date: 2023-09-06)

• Primary citation: Brownell, J.E.,Sintchak, M.D.,Gavin, J.M.,Liao, H.,Bruzzese, F.J.,Bump, N.J.,Soucy, T.A.,Milhollen, M.A.,Yang, X.,Burkhardt, A.L.,Ma, J.,Loke, H.K.,Lingaraj, T.,Wu, D.,Hamman, K.B.,Spelman, J.J.,Cullis, C.A.,Langston, S.P.,Vyskocil, S.,Sells, T.B.,Mallender, W.D.,Visiers, I.,Li, P.,Claiborne, C.F.,Rolfe, M.,Bolen, J.B.,Dick, L.R.; Substrate-assisted inhibition of ubiquitin-like protein-activating enzymes: the NEDD8 E1 inhibitor MLN4924 forms a NEDD8-AMP mimetic in situ.; Mol.Cell, 37:102-111, 2010; PubMed: 20129059; DOI: 10.1016/j.molcel.2009.12.024

Experimental method

X-RAY DIFFRACTION (3 Å)