3GPQ

Crystal structure of macro domain of Chikungunya virus in complex with RNA

Summary for 3GPQ

Related3GPG 3GPO 3GQE
DescriptorNon-structural protein 3, RNA (5'-R(*AP*AP*A)-3') (3 entities in total)
Functional Keywordsmacro domain, x domain, chikungunya, alphavirus, virus, vizier. viral enzymes involved in replication, rna, atp-binding, cell membrane, endosome, helicase, hydrolase, lipoprotein, lysosome, membrane, methyltransferase, mrna capping, mrna processing, multifunctional enzyme, nucleotide-binding, nucleotidyltransferase, nucleus, palmitate, phosphoprotein, protease, rna replication, rna-binding, rna-directed rna polymerase, thiol protease, transferase, viral protein/rna, viral protein-rna complex
Biological sourceChikungunya virus
Cellular locationNon-structural polyprotein: Host endosome membrane; Peripheral membrane protein; Cytoplasmic side (By similarity). P123: Host endosome membrane; Peripheral membrane protein; Cytoplasmic side (By similarity). mRNA-capping enzyme nsP1: Host endosome membrane; Peripheral membrane protein; Cytoplasmic side (By similarity). Protease nsP2: Host endosome membrane; Peripheral membrane protein; Cytoplasmic side (By similarity). Non-structural protein 3: Host endosome membrane; Peripheral membrane protein; Cytoplasmic side (By similarity). RNA-directed RNA polymerase nsP4: Host endosome membrane; Peripheral membrane protein; Cytoplasmic side (By similarity) Q8JUX6
Total number of polymer chains6
Total molecular weight76425.81
Authors
Malet, H.,Jamal, S.,Coutard, B.,Canard, B. (deposition date: 2009-03-23, release date: 2009-07-21, Last modification date: 2011-07-13)
Primary citation
Malet, H.,Coutard, B.,Jamal, S.,Dutartre, H.,Papageorgiou, N.,Neuvonen, M.,Ahola, T.,Forrester, N.,Gould, E.A.,Lafitte, D.,Ferron, F.,Lescar, J.,Gorbalenya, A.E.,de Lamballerie, X.,Canard, B.
The crystal structures of Chikungunya and Venezuelan equine encephalitis virus nsP3 macro domains define a conserved adenosine binding pocket
J.Virol., 83:6534-6545, 2009
PubMed: 19386706 (PDB entries with the same primary citation)
DOI: 10.1128/JVI.00189-09
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (2 Å)
?

Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliersRNA backbone0.2691606.6%20.4%0.00MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

Molmil generated image of 3gpq
no rotation
Molmil generated image of 3gpq
rotated about x axis by 90°
Molmil generated image of 3gpq
rotated about y axis by 90°