3GDH

Methyltransferase domain of human Trimethylguanosine Synthase 1 (TGS1) bound to m7GTP and adenosyl-homocysteine (active form)

3GDH の概要

• エントリーDOI: 10.2210/pdb3gdh/pdb
• 分子名称: Trimethylguanosine synthase homolog, 7-METHYL-GUANOSINE-5'-TRIPHOSPHATE, S-ADENOSYL-L-HOMOCYSTEINE, ... (6 entities in total)
• 機能のキーワード: m7g, cap, dimethyltransferase, usnrna, snorna, telomerase, cytoplasm, methyltransferase, nucleus, phosphoprotein, polymorphism, transcription, transcription regulation, transferase
• 由来する生物種: Homo sapiens (Human)
• 細胞内の位置: Cytoplasm : Q96RS0
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 83379.70

構造登録者

Monecke, T.,Dickmanns, A.,Ficner, R. (登録日: 2009-02-24, 公開日: 2009-06-23, 最終更新日: 2024-02-21)

主引用文献

Monecke, T.,Dickmanns, A.,Ficner, R.
Structural basis for m7G-cap hypermethylation of small nuclear, small nucleolar and telomerase RNA by the dimethyltransferase TGS1.
Nucleic Acids Res., 37:3865-3877, 2009

PubMed Abstract: The 5'-cap of spliceosomal small nuclear RNAs, some small nucleolar RNAs and of telomerase RNA was found to be hypermethylated in vivo. The Trimethylguanosine Synthase 1 (TGS1) mediates this conversion of the 7-methylguanosine-cap to the 2,2,7-trimethylguanosine (m(3)G)-cap during maturation of the RNPs. For mammalian UsnRNAs the generated m(2,2,7)G-cap is one part of a bipartite import signal mediating the transport of the UsnRNP-core complex into the nucleus. In order to understand the structural organization of human TGS1 as well as substrate binding and recognition we solved the crystal structure of the active TGS1 methyltransferase domain containing both, the minimal substrate m(7)GTP and the reaction product S-adenosyl-L-homocysteine (AdoHcy). The methyltransferase of human TGS1 harbors the canonical class 1 methyltransferase fold as well as an unique N-terminal, alpha-helical domain of 40 amino acids, which is essential for m(7)G-cap binding and catalysis. The crystal structure of the substrate bound methyltransferase domain as well as mutagenesis studies provide insight into the catalytic mechanism of TGS1.

PubMed: 19386620
DOI: 10.1093/nar/gkp249

実験手法

X-RAY DIFFRACTION (2 Å)