3G77

Bacterial cytosine deaminase V152A/F316C/D317G mutant

Summary for 3G77

Related1RB7 1RAK 1RA0 1R9Z 1R9Y 1K6W
DescriptorCytosine deaminase, FE (III) ION (3 entities in total)
Functional Keywordscytosine deaminase, protein engineering, cytosine metabolism, hydrolase, iron, metal-binding
Biological sourceEscherichia coli
Total number of polymer chains1
Total molecular weight47124.04
Authors
Stoddard, B.,Zhao, L. (deposition date: 2009-02-09, release date: 2009-09-22, Last modification date: 2011-07-13)
Primary citation
Fuchita, M.,Ardiani, A.,Zhao, L.,Serve, K.,Stoddard, B.L.,Black, M.E.
Bacterial cytosine deaminase mutants created by molecular engineering show improved 5-fluorocytosine-mediated cell killing in vitro and in vivo.
Cancer Res., 69:4791-4799, 2009
PubMed: 19487291 (PDB entries with the same primary citation)
DOI: 10.1158/0008-5472.CAN-09-0615
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (1.8 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.19150.2%1.4%2.1%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution
Download full validation report