3FCU

Structure of headpiece of integrin aIIBb3 in open conformation

3FCU の概要

• エントリーDOI: 10.2210/pdb3fcu/pdb
• 関連するPDBエントリー: 1TYE 2VDO 2VDP 2VDQ 2VDR 3FCS
• 分子名称: Integrin, alpha 2b, Integrin beta-3, alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (9 entities in total)
• 機能のキーワード: crystal structure; platelet integrin alphaiibbeta3; fibrinogen binding; allostery; therapeutic antagonism, cell adhesion, integrin, membrane, receptor, transmembrane, disease mutation, glycoprotein, host-virus interaction, phosphoprotein, cell adhesion-immune system complex, cell adhesion-blood clotting complex, cell adhesion/blood clotting
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 307075.08

構造登録者

Zhu, J.,Luo, B.-H.,Xiao, T.,Zhang, C.,Nishida, N.,Springer, T.A. (登録日: 2008-11-22, 公開日: 2009-01-20, 最終更新日: 2024-11-27)

主引用文献

Zhu, J.,Luo, B.H.,Xiao, T.,Zhang, C.,Nishida, N.,Springer, T.A.
Structure of a complete integrin ectodomain in a physiologic resting state and activation and deactivation by applied forces.
Mol.Cell, 32:849-861, 2008

PubMed Abstract: The complete ectodomain of integrin alpha(IIb)beta(3) reveals a bent, closed, low-affinity conformation, the beta knee, and a mechanism for linking cytoskeleton attachment to high affinity for ligand. Ca and Mg ions in the recognition site, including the synergistic metal ion binding site (SyMBS), are loaded prior to ligand binding. Electrophilicity of the ligand-binding Mg ion is increased in the open conformation. The beta(3) knee passes between the beta(3)-PSI and alpha(IIb)-knob to bury the lower beta leg in a cleft, from which it is released for extension. Different integrin molecules in crystals and EM reveal breathing that appears on pathway to extension. Tensile force applied to the extended ligand-receptor complex stabilizes the closed, low-affinity conformation. By contrast, an additional lateral force applied to the beta subunit to mimic attachment to moving actin filaments stabilizes the open, high-affinity conformation. This mechanism propagates allostery over long distances and couples cytoskeleton attachment of integrins to their high-affinity state.

PubMed: 19111664
DOI: 10.1016/j.molcel.2008.11.018

実験手法

X-RAY DIFFRACTION (2.9 Å)