3FBD

Crystal structure of the nuclease domain of COLE7(D493Q mutant) in complex with an 18-BP duplex DNA

Summary for 3FBD

• Entry DOI: 10.2210/pdb3fbd/pdb
• Related: 1PT3 1ZNS 2IVH
• Descriptor: Colicin-E7, 5'-D(*DGP*DGP*DAP*DAP*DTP*DTP*DCP*DGP*DAP*DTP*DCP*DGP*DAP*DAP*DTP*DTP*DCP*DC)-3' (3 entities in total)
• Functional Keywords: computational redesign, protein engineering, protein-nucleic acid interactions, dnase, dna hydrolysis., antibiotic, antimicrobial, bacteriocin, endonuclease, hydrolase, metal-binding, nuclease, plasmid, zinc, hydrolase-dna complex, hydrolase/dna
• Biological source: Escherichia coli
• Total number of polymer chains: 6
• Total formula weight: 52386.81

Authors

Wang, Y.T.,Doudeva, L.G.,Yuan, H.S. (deposition date: 2008-11-19, release date: 2009-11-03, Last modification date: 2023-11-01)

• Primary citation: Wang, Y.T.,Wright, J.D.,Doudeva, L.G.,Jhang, H.C.,Lim, C.,Yuan, H.S.; Redesign of high-affinity nonspecific nucleases with altered sequence preference; To be Published,

Experimental method

X-RAY DIFFRACTION (2.9 Å)