3F74

Crystal structure of wild type LFA1 I domain

3F74 の概要

• エントリーDOI: 10.2210/pdb3f74/pdb
• 関連するPDBエントリー: 3F78
• 分子名称: Integrin alpha-L, GLYCEROL, MAGNESIUM ION, ... (4 entities in total)
• 機能のキーワード: integrin, lfa1, i domain, inactive conformation, wild type, cell adhesion, glycoprotein, magnesium, membrane, receptor, transmembrane
• 由来する生物種: Homo sapiens (Human)
• 細胞内の位置: Cell membrane ; Single-pass type I membrane protein : P20701
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 62427.79

構造登録者

Zhang, H.,Wang, J.-H. (登録日: 2008-11-07, 公開日: 2009-06-23, 最終更新日: 2023-12-27)

主引用文献

Zhang, H.,Astrof, N.S.,Liu, J.H.,Wang, J.H.,Shimaoka, M.
Crystal structure of isoflurane bound to integrin LFA-1 supports a unified mechanism of volatile anesthetic action in the immune and central nervous systems.
Faseb J., 23:2735-2740, 2009

PubMed Abstract: Volatile anesthetics (VAs), such as isoflurane, induce a general anesthetic state by binding to specific targets (i.e., ion channels) in the central nervous system (CNS). Simultaneously, VAs modulate immune functions, possibly via direct interaction with alternative targets on leukocytes. One such target, the integrin lymphocyte function-associated antigen-1 (LFA-1), has been shown previously to be inhibited by isoflurane. A better understanding of the mechanism by which isoflurane alters protein function requires the detailed information about the drug-protein interaction at an atomic level. Here, we describe the crystal structure of the LFA-1 ligand-binding domain (I domain) in complex with isoflurane at 1.6 A. We discovered that isoflurane binds to an allosteric cavity previously implicated as critical for the transition of LFA-1 from the low- to the high-affinity state. The isoflurane binding site in the I domain involves an array of amphiphilic interactions, thereby resembling a "common anesthetic binding motif" previously predicted for authentic VA binding sites. These results suggest that the allosteric modulation of protein function by isoflurane, as demonstrated for the integrin LFA-1, might represent a unified mechanism shared by the interactions of volatile anesthetics with targets in the CNS.

PubMed: 19332643
DOI: 10.1096/fj.09-129908

実験手法

X-RAY DIFFRACTION (1.7 Å)