3F06

Crystal Structure Analysis of Human HDAC8 D101A Variant.

Summary for 3F06

• Entry DOI: 10.2210/pdb3f06/pdb
• Related: 3EW8 3EZP 3EZT 3F07 3F0R
• Descriptor: Histone deacetylase 8, ZINC ION, POTASSIUM ION, ... (6 entities in total)
• Functional Keywords: hydrolase, hdac, metalloenzyme, arginase fold, hdac8, histone deacetylase 8, hydroxamate inhibitor, mutant, chromatin regulator, nucleus, repressor, transcription, transcription regulation
• Biological source: Homo sapiens (Human)
• Cellular location: Nucleus: Q9BY41
• Total number of polymer chains: 2
• Total formula weight: 87320.12

Authors

Dowling, D.P.,Gantt, S.L.,Gattis, S.G.,Fierke, C.A.,Christianson, D.W. (deposition date: 2008-10-24, release date: 2008-12-30, Last modification date: 2023-09-06)

Primary citation

Dowling, D.P.,Gantt, S.L.,Gattis, S.G.,Fierke, C.A.,Christianson, D.W.
Structural studies of human histone deacetylase 8 and its site-specific variants complexed with substrate and inhibitors.
Biochemistry, 47:13554-13563, 2008

PubMed Abstract: Metal-dependent histone deacetylases (HDACs) require Zn(2+) or Fe(2+) to regulate the acetylation of lysine residues in histones and other proteins in eukaryotic cells. Isozyme HDAC8 is perhaps the archetypical member of the class I HDAC family and serves as a paradigm for studying structure-function relationships. Here, we report the structures of HDAC8 complexes with trichostatin A and 3-(1-methyl-4-phenylacetyl-1H-2-pyrrolyl)-N-hydroxy-2-propenamide (APHA) in a new crystal form. The structure of the APHA complex reveals that the hydroxamate CO group accepts a hydrogen bond from Y306 but does not coordinate to Zn(2+) with favorable geometry, perhaps due to the constraints of its extended pi system. Additionally, since APHA binds to only two of the three protein molecules in the asymmetric unit of this complex, the structure of the third monomer represents the first structure of HDAC8 in the unliganded state. Comparison of unliganded and liganded structures illustrates ligand-induced conformational changes in the L2 loop that likely accompany substrate binding and catalysis. Furthermore, these structures, along with those of the D101N, D101E, D101A, and D101L variants, support the proposal that D101 is critical for the function of the L2 loop. However, amino acid substitutions for D101 can also trigger conformational changes of Y111 and W141 that perturb the substrate binding site. Finally, the structure of H143A HDAC8 complexed with an intact acetylated tetrapeptide substrate molecule confirms the importance of D101 for substrate binding and reveals how Y306 and the active site zinc ion together bind and activate the scissile amide linkage of acetyllysine.

PubMed: 19053282
DOI: 10.1021/bi801610c

Experimental method

X-RAY DIFFRACTION (2.55 Å)