3EZM

CYANOVIRIN-N

Summary for 3EZM

• Entry DOI: 10.2210/pdb3ezm/pdb
• Descriptor: PROTEIN (CYANOVIRIN-N) (2 entities in total)
• Functional Keywords: cyanovirin-n, hiv-inactivating, domain-swapping, gp120, immune system
• Biological source: Nostoc ellipsosporum
• Total number of polymer chains: 1
• Total formula weight: 11022.09

Authors

Yang, F.,Wlodawer, A. (deposition date: 1998-12-15, release date: 1998-12-23, Last modification date: 2023-09-06)

Primary citation

Yang, F.,Bewley, C.A.,Louis, J.M.,Gustafson, K.R.,Boyd, M.R.,Gronenborn, A.M.,Clore, G.M.,Wlodawer, A.
Crystal structure of cyanovirin-N, a potent HIV-inactivating protein, shows unexpected domain swapping.
J.Mol.Biol., 288:403-412, 1999

PubMed Abstract: The crystal structure of cyanovirin-N (CV-N), a protein with potent antiviral activity, was solved at 1.5 A resolution by molecular replacement using as the search model the solution structure previously determined by NMR. The crystals belong to the space group P3221 with one monomer of CV-N in each asymmetric unit. The primary structure of CV-N contains 101 residues organized in two domains, A (residues 1 to 50) and B (residues 51 to 101), with a high degree of internal sequence and structural similarity. We found that under the conditions of the crystallographic experiments (low pH and 26 % isopropanol), two symmetrically related monomers form a dimer by domain swapping, such that domain A of one monomer interacts with domain B' of its crystallographic symmetry mate and vice versa. Because the two swapped domains are distant from each other, domain swapping does not result in additional intramolecular interactions. Even though one of the protein sample solutions that was used for crystallization clearly contained 100 % monomeric CV-N molecules, as judged by various methods, we were only able to obtain crystals containing domain-swapped dimers. With the exception of the unexpected phenomenon of domain swapping, the crystal structure of CV-N is very similar to the NMR structure, with a root-mean-square deviation of 0.55 A for the main-chain atoms, the best agreement reported to date for structures solved using both techniques.

PubMed: 10329150
DOI: 10.1006/jmbi.1999.2693

Experimental method

X-RAY DIFFRACTION (1.5 Å)