3EZM
CYANOVIRIN-N
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F2 |
| Synchrotron site | CHESS |
| Beamline | F2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-08 |
| Detector | ADSC |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 48.068, 48.068, 79.080 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 1.500 |
| R-factor | 0.184 * |
| Rwork | 0.184 |
| R-free | 0.20600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ezm |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (0.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 1.540 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.058 * | 0.368 * |
| Total number of observations | 107021 * | |
| Number of reflections | 17537 | |
| <I/σ(I)> | 9.5 | |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4.6 * | 26% ISOPROPANOL, 0.1M SODIUM ACETATE, 0.15M CALCIUM CHLORIDE, 5% PEG 400, PH 4.4 |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | ISOPROPANOL | ||
| 2 | 1 | 1 | SODIUM ACETATE | ||
| 3 | 1 | 1 | PEG 400 | ||
| 4 | 1 | 1 | CACL2 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | isopropanol | 20 (%(v/v)) | |
| 2 | 1 | drop | sodium acetate | 0.1 (M) | |
| 3 | 1 | drop | 0.2 (M) | ||
| 4 | 1 | reservoir | isopropanol | 26 (%(v/v)) | |
| 5 | 1 | reservoir | sodium acetate | 0.1 (M) | |
| 6 | 1 | reservoir | 0.15 (M) | ||
| 7 | 1 | reservoir | PEG400 | 5 (%(v/v)) |
