3ER9

Crystal structure of the heterodimeric vaccinia virus mRNA polyadenylate polymerase complex with UU and 3'-deoxy ATP

3ER9 の概要

• エントリーDOI: 10.2210/pdb3er9/pdb
• 関連するPDBエントリー: 2GA9 3ER8 3ERC
• 分子名称: Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase, Poly(A) polymerase catalytic subunit, 5'-R(UP*U)-3', ... (7 entities in total)
• 機能のキーワード: polyadenylate polymerase, translocation, single tranded rna poly(a) polymerase, rna protein complex, processivity, heterodimer, nucleotidyltransferase, poxvirus; methyltransferase, mrna capping, mrna processing, s-adenosyl-l-methionine, transcription, transferase, transferase-rna complex, transferase/rna
• 由来する生物種: vaccinia virus WR; 詳細
• 細胞内の位置: Virion : P07617
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 91578.08

構造登録者

Li, C.,Li, H.,Zhou, S.,Poulos, T.L.,Gershon, P.D. (登録日: 2008-10-01, 公開日: 2009-06-16, 最終更新日: 2023-12-27)

主引用文献

Li, C.,Li, H.,Zhou, S.,Sun, E.,Yoshizawa, J.,Poulos, T.L.,Gershon, P.D.
Polymerase Translocation with Respect to Single-Stranded Nucleic Acid: Looping or Wrapping of Primer around a Poly(A) Polymerase
Structure, 17:680-689, 2009

PubMed Abstract: Vaccinia virus protein VP55 translocates continuously with respect to single-stranded nucleic acid while extending its 3'end. Here, all key sites of polymerase-primer interaction were identified, demonstrating the wrapping or looping of polyadenylation primer around the polymerase during translocation. Side-chain substitutions at one of the sites indicated its requirement for tail extension beyond approximately 12 nucleotides in length, and conformational changes observed upon oligonucleotide binding suggested allosteric connectivity during translocation. Conformational changes in VP39 upon VP55 binding suggested that, within the VP55-VP39 complex, VP39's mRNA 5' cap binding site closes. The crystallographic structure showed a PAPase catalytic center without side-chain substitutions, possessing two metal ions and with all known reactive and catalytic groups represented, fitting a classical two-metal ion mechanism for phosphoryl transfer.

PubMed: 19446524
DOI: 10.1016/j.str.2009.03.012

実験手法

X-RAY DIFFRACTION (2.06 Å)