3EIG

Crystal structure of a methotrexate-resistant mutant of human dihydrofolate reductase

Summary for 3EIG

Entry DOI	10.2210/pdb3eig/pdb
Descriptor	Dihydrofolate reductase, METHOTREXATE, SULFATE ION, ... (5 entities in total)
Functional Keywords	oxidoreductase, mutant human dihydrofolale reductase, nadp, one-carbon metabolism
Biological source	Homo sapiens (Human)
Total number of polymer chains	1
Total formula weight	22695.88
Authors	Yachnin, B.J.,Berghuis, A.M. (deposition date: 2008-09-15, release date: 2009-05-26, Last modification date: 2023-08-30)
Primary citation	Volpato, J.P.,Yachnin, B.J.,Blanchet, J.,Guerrero, V.,Poulin, L.,Fossati, E.,Berghuis, A.M.,Pelletier, J.N. Multiple conformers in active site of human dihydrofolate reductase F31R/Q35E double mutant suggest structural basis for methotrexate resistance. J.Biol.Chem., 284:20079-20089, 2009 Cited by PubMed: 19478082 DOI: 10.1074/jbc.M109.018010 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (1.7 Å)

Structure validation