3EIG
Crystal structure of a methotrexate-resistant mutant of human dihydrofolate reductase
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A | Dihydrofolate reductase | polymer | 186 | 21360.5 | 1 | UniProt (P00374) Pfam (PF00186) In PDB | Homo sapiens (Human) | |
| 2 | A | METHOTREXATE | non-polymer | 454.4 | 1 | Chemie (MTX) | |||
| 3 | A | SULFATE ION | non-polymer | 96.1 | 8 | Chemie (SO4) | |||
| 4 | A | CADMIUM ION | non-polymer | 112.4 | 1 | Chemie (CD) | |||
| 5 | water | water | 18.0 | 138 | Chemie (HOH) |
Sequence modifications
A: 1 - 186 (UniProt: P00374)
| PDB | External Database | Details |
|---|---|---|
| Arg 31 | Phe 32 | engineered mutation |
| Glu 35 | Gln 36 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 21360.5 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 1335.4 | |
| All* | Total formula weight | 22695.9 |
