3EI4

Structure of the hsDDB1-hsDDB2 complex

Summary for 3EI4

• Entry DOI: 10.2210/pdb3ei4/pdb
• Related: 3EI1 3EI2 3EI3
• Descriptor: DNA damage-binding protein 1, DNA damage-binding protein 2 (2 entities in total)
• Functional Keywords: uv-damage; ddb, nucleotide excision repair, xeroderma pigmentosum, disease mutation, dna damage, dna repair, dna-binding, nucleus, phosphoprotein, wd repeat, dna binding protein
• Biological source: Homo sapiens (Human); More
• Cellular location: Cytoplasm: Q16531; Nucleus : Q92466
• Total number of polymer chains: 6
• Total formula weight: 534963.19

Authors

Scrima, A.,Pavletich, N.P.,Thoma, N.H. (deposition date: 2008-09-15, release date: 2009-01-20, Last modification date: 2024-03-20)

Primary citation

Scrima, A.,Konickova, R.,Czyzewski, B.K.,Kawasaki, Y.,Jeffrey, P.D.,Groisman, R.,Nakatani, Y.,Iwai, S.,Pavletich, N.P.,Thoma, N.H.
Structural basis of UV DNA-damage recognition by the DDB1-DDB2 complex.
Cell(Cambridge,Mass.), 135:1213-1223, 2008

PubMed Abstract: Ultraviolet (UV) light-induced pyrimidine photodimers are repaired by the nucleotide excision repair pathway. Photolesions have biophysical parameters closely resembling undamaged DNA, impeding discovery through damage surveillance proteins. The DDB1-DDB2 complex serves in the initial detection of UV lesions in vivo. Here we present the structures of the DDB1-DDB2 complex alone and bound to DNA containing either a 6-4 pyrimidine-pyrimidone photodimer (6-4PP) lesion or an abasic site. The structure shows that the lesion is held exclusively by the WD40 domain of DDB2. A DDB2 hairpin inserts into the minor groove, extrudes the photodimer into a binding pocket, and kinks the duplex by approximately 40 degrees. The tightly localized probing of the photolesions, combined with proofreading in the photodimer pocket, enables DDB2 to detect lesions refractory to detection by other damage surveillance proteins. The structure provides insights into damage recognition in chromatin and suggests a mechanism by which the DDB1-associated CUL4 ubiquitin ligase targets proteins surrounding the site of damage.

PubMed: 19109893
DOI: 10.1016/j.cell.2008.10.045

Experimental method

X-RAY DIFFRACTION (3.3 Å)