3EBC

Structure of N141A HincII with Cognate DNA

Summary for 3EBC

• Entry DOI: 10.2210/pdb3ebc/pdb
• Descriptor: Type-2 restriction enzyme HincII, 5'-D(*DGP*DCP*DCP*DGP*DGP*DTP*DCP*DGP*DAP*DCP*DGP*DGP*DGP*DC)-3', 5'-D(*DGP*DCP*DCP*DCP*DGP*DTP*DCP*DGP*DAP*DCP*DCP*DGP*DGP*DC)-3', ... (5 entities in total)
• Functional Keywords: intermediate, mutant, dimerization, r-loop, endonuclease, hydrolase, nuclease, restriction system, hydrolase-dna complex, hydrolase/dna
• Biological source: Haemophilus influenzae
• Total number of polymer chains: 4
• Total formula weight: 81807.46

Authors

Little, E.J.,Babic, A.C.,Horton, N.C. (deposition date: 2008-08-27, release date: 2008-12-23, Last modification date: 2024-02-21)

Primary citation

Little, E.J.,Babic, A.C.,Horton, N.C.
Early Interrogation and Recognition of DNA Sequence by Indirect Readout
Structure, 16:1828-1837, 2008

PubMed Abstract: Control of replication, transcription, recombination and repair requires proteins capable of finding particular DNA sequences in a background of a large excess of nonspecific sequences. Such recognition can involve direct readout, with direct contacts to the bases of DNA, or in some cases through the less well-characterized indirect readout mechanisms. In order to measure the relative contributions of direct and indirect readout by a sequence specific endonuclease, HincII, a mutant enzyme deficient in a direct contact, was characterized, and surprisingly showed no loss of sequence specificity. The three dimensional crystal structure shows the loss of most of the direct readout contacts to the DNA, possibly capturing an early stage in target site recognition using predominately indirect readout to prescreen sites before full sequence interrogation.

PubMed: 19081059

Experimental method

X-RAY DIFFRACTION (2.55 Å)