3EBC
Structure of N141A HincII with Cognate DNA
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Type-2 restriction enzyme HincII | polymer | 317 | 36593.5 | 2 | UniProt (P17743) Pfam (PF09226) | Haemophilus influenzae | R.HincII, Type II restriction enzyme HincII, Endonuclease HincII |
| 2 | C (E) | 5'-D(*DGP*DCP*DCP*DGP*DGP*DTP*DCP*DGP*DAP*DCP*DGP*DGP*DGP*DC)-3' | polymer | 14 | 4322.8 | 1 | |||
| 3 | D (F) | 5'-D(*DGP*DCP*DCP*DCP*DGP*DTP*DCP*DGP*DAP*DCP*DCP*DGP*DGP*DC)-3' | polymer | 14 | 4242.7 | 1 | |||
| 4 | E (A) | MANGANESE (II) ION | non-polymer | 54.9 | 1 | Chemie (MN) | |||
| 5 | F, G, H, I (A, B, E, F) | water | water | 18.0 | 301 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 258 (UniProt: P17743)
| PDB | External Database | Details |
|---|---|---|
| Met -1 | - | expression tag |
| Ala 0 | - | expression tag |
| Thr 130 | Arg 130 | conflict |
| Ala 141 | Asn 141 | engineered mutation |
| Trp 173 | Ser 173 | conflict |
| Arg 259 | - | expression tag |
| Ser 260 | - | expression tag |
| Arg 261 | - | expression tag |
| Glu 262 | - | expression tag |
| Leu 263 | - | expression tag |
| Val 264 | - | expression tag |
| Asp 265 | - | expression tag |
| Pro 266 | - | expression tag |
| Asn 267 | - | expression tag |
| Ser 268 | - | expression tag |
| Val 269 | - | expression tag |
| Gln 270 | - | expression tag |
| Ala 271 | - | expression tag |
| Arg 272 | - | expression tag |
| Leu 273 | - | expression tag |
| Gln 274 | - | expression tag |
| Asp 275 | - | expression tag |
| Val 276 | - | expression tag |
| Asp 277 | - | expression tag |
| Gly 278 | - | expression tag |
| Thr 279 | - | expression tag |
| Ile 280 | - | expression tag |
| Asp 281 | - | expression tag |
| Thr 282 | - | expression tag |
| Arg 283 | - | expression tag |
| Ser 284 | - | expression tag |
| Lys 285 | - | expression tag |
| Leu 286 | - | expression tag |
| Ala 287 | - | expression tag |
| Ala 288 | - | expression tag |
| Ala 289 | - | expression tag |
| Gln 290 | - | expression tag |
| Leu 291 | - | expression tag |
| Tyr 292 | - | expression tag |
| Thr 293 | - | expression tag |
| Arg 294 | - | expression tag |
| Ala 295 | - | expression tag |
| Ser 296 | - | expression tag |
| Gln 297 | - | expression tag |
| Pro 298 | - | expression tag |
| Glu 299 | - | expression tag |
| Leu 300 | - | expression tag |
| Ala 301 | - | expression tag |
| Pro 302 | - | expression tag |
| Glu 303 | - | expression tag |
| Asp 304 | - | expression tag |
| Pro 305 | - | expression tag |
| Glu 306 | - | expression tag |
| Asp 307 | - | expression tag |
| Leu 308 | - | expression tag |
| Glu 309 | - | expression tag |
| His 310 | - | expression tag |
| His 311 | - | expression tag |
| His 312 | - | expression tag |
| His 313 | - | expression tag |
| His 314 | - | expression tag |
| His 315 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 81752.5 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 54.9 | |
| All* | Total formula weight | 81807.5 |
