3EBC
Structure of N141A HincII with Cognate DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.551, 91.571, 70.594 |
| Unit cell angles | 90.00, 107.46, 90.00 |
Refinement procedure
| Resolution | 43.560 - 2.550 |
| R-factor | 0.195 |
| Rwork | 0.191 |
| R-free | 0.27900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.380 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.560 | |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.093 | 0.313 |
| Number of reflections | 17810 | |
| <I/σ(I)> | 1.9 | 1.9 |
| Completeness [%] | 96.1 | 96.1 |
| Redundancy | 3.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 290.15 | 22% PEG 4000, 0.1M imidazole buffer, 0.2M NaCl, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 290.15K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 4000 | ||
| 2 | 1 | 1 | imidazole buffer | ||
| 3 | 1 | 1 | NaCl | ||
| 4 | 1 | 2 | PEG 4000 | ||
| 5 | 1 | 2 | imidazole buffer | ||
| 6 | 1 | 2 | NaCl |
