3EBC
Structure of N141A HincII with Cognate DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.3 |
Synchrotron site | ALS |
Beamline | 5.0.3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-10-12 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 46.551, 91.571, 70.594 |
Unit cell angles | 90.00, 107.46, 90.00 |
Refinement procedure
Resolution | 43.560 - 2.550 |
R-factor | 0.195 |
Rwork | 0.191 |
R-free | 0.27900 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.380 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.560 | |
High resolution limit [Å] | 2.550 | 2.550 |
Rmerge | 0.093 | 0.313 |
Number of reflections | 17810 | |
<I/σ(I)> | 1.9 | 1.9 |
Completeness [%] | 96.1 | 96.1 |
Redundancy | 3.4 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 290.15 | 22% PEG 4000, 0.1M imidazole buffer, 0.2M NaCl, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 290.15K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 4000 | ||
2 | 1 | 1 | imidazole buffer | ||
3 | 1 | 1 | NaCl | ||
4 | 1 | 2 | PEG 4000 | ||
5 | 1 | 2 | imidazole buffer | ||
6 | 1 | 2 | NaCl |