3E2U

Crystal structure of the zink-knuckle 2 domain of human CLIP-170 in complex with CAP-Gly domain of human dynactin-1 (p150-GLUED)

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3E2U の概要

• エントリーDOI: 10.2210/pdb3e2u/pdb
• 関連するPDBエントリー: 1TXQ 2CP5 2HKN 2HKQ 2HQH
• 分子名称: Dynactin subunit 1, CAP-Gly domain-containing linker protein 1, ZINC ION, ... (4 entities in total)
• 機能のキーワード: structural protein microtubule binding, dynactin, cytoskeleton associated protein, p150glued, clip-170/restin, +tip protein complex structure, zinc-knuckle, autoinhibition, protein binding, cytoskeleton, dynein, microtubule, motor protein, phosphoprotein
• 由来する生物種: Homo sapiens (Human); 詳細
• 細胞内の位置: Cytoplasm: Q14203 P30622
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 60715.70

構造登録者

Weisbrich, A.,Honnappa, S.,Capitani, G.,Steinmetz, M.O. (登録日: 2008-08-06, 公開日: 2008-08-19, 最終更新日: 2023-11-01)

主引用文献

Weisbrich, A.,Honnappa, S.,Jaussi, R.,Okhrimenko, O.,Frey, D.,Jelesarov, I.,Akhmanova, A.,Steinmetz, M.O.
Structure-function relationship of CAP-Gly domains
Nat.Struct.Mol.Biol., 14:959-967, 2007

PubMed Abstract: In all eukaryotes, CAP-Gly proteins control important cellular processes. The molecular mechanisms underlying the functions of CAP-Gly domains, however, are still poorly understood. Here we use the complex formed between the CAP-Gly domain of p150(glued) and the C-terminal zinc knuckle of CLIP170 as a model system to explore the structure-function relationship of CAP-Gly-mediated protein interactions. We demonstrate that the conserved GKNDG motif of CAP-Gly domains is responsible for targeting to the C-terminal EEY/F sequence motifs of CLIP170, EB proteins and microtubules. The CAP-Gly-EEY/F interaction is essential for the recruitment of the dynactin complex by CLIP170 and for activation of CLIP170. Our findings define the molecular basis of CAP-Gly domain function, including the tubulin detyrosination-tyrosination cycle. They further establish fundamental roles for the interaction between CAP-Gly proteins and C-terminal EEY/F sequence motifs in regulating complex and dynamic cellular processes.

PubMed: 17828277
DOI: 10.1038/nsmb1291

実験手法

X-RAY DIFFRACTION (2.6 Å)