3E2U

Crystal structure of the zink-knuckle 2 domain of human CLIP-170 in complex with CAP-Gly domain of human dynactin-1 (p150-GLUED)

Replaces:  2PZO

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	CCD
Collection date	2006-10-26
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.039
Spacegroup name	C 2 2 21
Unit cell lengths	116.220, 116.310, 79.880
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.015 - 2.600
R-factor	0.193
Rwork	0.191
R-free	0.23800
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	2PZO (SOLVED BY MOLECULAR REPLACEMENT USING MOLREP)
RMSD bond length	0.007
RMSD bond angle	1.103
Data reduction software	XDS
Data scaling software	XSCALE
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.020	2.800
High resolution limit [Å]	2.600	2.600
Number of reflections	16917	3307
<I/σ(I)>	10	3.3
Completeness [%]	99.5	99.7
Redundancy	5.2	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	1.2M Trisodium Citrate, 0.1M HEPES, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K