2HQH

Crystal structure of p150Glued and CLIP-170

Summary for 2HQH

• Entry DOI: 10.2210/pdb2hqh/pdb
• Related: 1TXQ
• Descriptor: Dynactin-1, Restin, ZINC ION, ... (4 entities in total)
• Functional Keywords: beta/beta structure, zinc finger motif, structural protein, protein binding
• Biological source: Homo sapiens (human); More
• Cellular location: Cytoplasm : Q14203 P30622
• Total number of polymer chains: 8
• Total formula weight: 51686.63

Authors

Hayashi, I.,Ikura, M. (deposition date: 2006-07-18, release date: 2007-08-21, Last modification date: 2024-02-14)

Primary citation

Hayashi, I.,Plevin, M.J.,Ikura, M.
CLIP170 autoinhibition mimics intermolecular interactions with p150Glued or EB1.
Nat.Struct.Mol.Biol., 14:980-981, 2007

PubMed Abstract: CLIP170 and p150(Glued) localize to the plus ends of growing microtubules. Using crystallography and NMR, we show that autoinhibitory interactions within CLIP170 use the same binding determinants as CLIP170's intermolecular interactions with p150(Glued). These interactions have both similar and distinct features when compared with the p150(Glued)-EB1 complex. Our data thus demonstrate that regulation of microtubule dynamics by plus end-tracking proteins (+TIPs) occurs through direct competition between homologous binding interfaces.

PubMed: 17828275
DOI: 10.1038/nsmb1299

Experimental method

X-RAY DIFFRACTION (1.8 Å)