3DYS

human phosphodiestrase-5'GMP complex (EP), produced by soaking with 20mM cGMP+20 mM MnCl2+20 mM MgCl2 for 2 hours, and flash-cooled to liquid nitrogen temperature when substrate was still abudant.

Summary for 3DYS

Entry DOI	10.2210/pdb3dys/pdb
Related	3DY8 3DYL 3DYN 3DYQ
Descriptor	High affinity cGMP-specific 3',5'-cyclic phosphodiesterase 9A, MANGANESE (II) ION, MAGNESIUM ION, ... (7 entities in total)
Functional Keywords	phosphodiestrase, enzyme mechanism, cgmp, hydrolase, manganese, metal-binding, phosphoprotein
Biological source	Homo sapiens (Human)
Cellular location	Isoform PDE9A1: Cell projection, ruffle membrane. Isoform PDE9A2: Cell projection, ruffle membrane. Isoform PDE9A3: Cytoplasm. Isoform PDE9A17: Cytoplasm: O76083
Total number of polymer chains	2
Total formula weight	78625.70
Authors	Liu, S.,Mansour, M.N.,Dillman, K.,Perez, J.,Danley, D.,Menniti, F. (deposition date: 2008-07-28, release date: 2008-09-16, Last modification date: 2024-02-21)
Primary citation	Liu, S.,Mansour, M.N.,Dillman, K.S.,Perez, J.R.,Danley, D.E.,Aeed, P.A.,Simons, S.P.,Lemotte, P.K.,Menniti, F.S. Structural basis for the catalytic mechanism of human phosphodiesterase 9. Proc.Natl.Acad.Sci.Usa, 105:13309-13314, 2008 Cited by PubMed: 18757755 DOI: 10.1073/pnas.0708850105 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.3 Å)

Structure validation