3DYS
human phosphodiestrase-5'GMP complex (EP), produced by soaking with 20mM cGMP+20 mM MnCl2+20 mM MgCl2 for 2 hours, and flash-cooled to liquid nitrogen temperature when substrate was still abudant.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | High affinity cGMP-specific 3',5'-cyclic phosphodiesterase 9A | polymer | 329 | 38862.9 | 2 | UniProt (O76083) Pfam (PF00233) | Homo sapiens (Human) | |
| 2 | C, E, G, I (A, B) | MANGANESE (II) ION | non-polymer | 54.9 | 4 | Chemie (MN) | |||
| 3 | D, H (A, B) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 4 | F (A) | GUANOSINE-5'-MONOPHOSPHATE | non-polymer | 363.2 | 1 | Chemie (5GP) | |||
| 5 | J (B) | 3-ISOBUTYL-1-METHYLXANTHINE | non-polymer | 222.2 | 1 | Chemie (IBM) | |||
| 6 | K (B) | FORMIC ACID | non-polymer | 46.0 | 1 | Chemie (FMT) | |||
| 7 | L, M (A, B) | water | water | 18.0 | 463 | Chemie (HOH) |
Sequence modifications
A, B: 182 - 506 (UniProt: O76083)
| PDB | External Database | Details |
|---|---|---|
| Gly 178 | - | expression tag |
| Ser 179 | - | expression tag |
| His 180 | - | expression tag |
| Met 181 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 77725.9 | |
| Non-Polymers* | Number of molecules | 9 |
| Total formula weight | 899.9 | |
| All* | Total formula weight | 78625.7 |
