3DP5
Crystal structure of Geobacter sulfurreducens OmcF with N-terminal Strep-tag II
Summary for 3DP5
| Entry DOI | 10.2210/pdb3dp5/pdb |
| Descriptor | Cytochrome c family protein, SULFATE ION, HEME C, ... (4 entities in total) |
| Functional Keywords | c-type cytochrome, fe sad phasing, dissimilatory metal reduction, geobacter sulfurreducens, electron transport |
| Biological source | Geobacter sulfurreducens |
| Total number of polymer chains | 1 |
| Total formula weight | 11066.18 |
| Authors | Lukat, P.,Hoffmann, M.,Einsle, O. (deposition date: 2008-07-07, release date: 2008-09-02, Last modification date: 2024-10-30) |
| Primary citation | Lukat, P.,Hoffmann, M.,Einsle, O. Crystal packing of the c(6)-type cytochrome OmcF from Geobacter sulfurreducens is mediated by an N-terminal Strep-tag II ACTA CRYSTALLOGR.,SECT.D, 64:919-926, 2008 Cited by PubMed Abstract: The putative outer membrane c-type cytochrome OmcF from Geobacter sulfurreducens contains a single haem group and shows homology to soluble cytochromes c(6), a class of electron-transfer proteins that are typically found in cyanobacterial photosynthetic electron-transfer chains. OmcF was overexpressed heterologously in Escherichia coli as an N-terminal Strep-tag II fusion protein and isolated using streptactin-affinity chromatography followed by size-exclusion chromatography. The structure was solved by Fe SAD using data collected to a resolution of 1.86 A on a rotating copper-anode X-ray generator. In the crystal, packing interactions in one dimension were exclusively mediated through the Strep-tag II sequence. The tag and linker regions were in contact with three further monomers of OmcF, leading to a well defined electron-density map for this engineered and secondary-structure-free region of the molecule. PubMed: 18703839DOI: 10.1107/S0907444908021306 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.86 Å) |
Structure validation
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