3DP5
Crystal structure of Geobacter sulfurreducens OmcF with N-terminal Strep-tag II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-02-15 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 53.475, 40.125, 42.085 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.080 - 1.860 |
| R-factor | 0.17236 |
| Rwork | 0.170 |
| R-free | 0.20987 |
| Structure solution method | SAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.776 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.080 | 1.960 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Rmerge | 0.495 | |
| Number of reflections | 8066 | |
| <I/σ(I)> | 23.5 | 6.06 |
| Completeness [%] | 99.7 | 98.5 |
| Redundancy | 21.78 | 16.04 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 1.4-1.8M ammonium sulfate, 2% PEG400, 100mM HEPES/NaOH, pH7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
