3DM7

Crystal Structure of the Vps75 Histone Chaperone

Summary for 3DM7

• Entry DOI: 10.2210/pdb3dm7/pdb
• Related: 3D35
• Descriptor: Vacuolar protein sorting-associated protein 75 (2 entities in total)
• Functional Keywords: vps75 (vacuolar protein sorting 75), nap1, histone chaperone, nucleus, phosphoprotein, protein transport, transport, chaperone
• Biological source: Saccharomyces cerevisiae (brewer's yeast,lager beer yeast,yeast)
• Cellular location: Nucleus: P53853
• Total number of polymer chains: 2
• Total formula weight: 55602.18

Authors

Tang, Y.,Marmorstein, R. (deposition date: 2008-06-30, release date: 2008-09-09, Last modification date: 2024-10-30)

Primary citation

Tang, Y.,Meeth, K.,Jiang, E.,Luo, C.,Marmorstein, R.
Structure of Vps75 and implications for histone chaperone function.
Proc.Natl.Acad.Sci.Usa, 105:12206-12211, 2008

PubMed Abstract: The vacuolar protein sorting 75 (Vps75) histone chaperone participates in chromatin assembly and disassembly at both active and inactive genes through the preferential binding to histone H3-H4. Vps75 is also one of two histone chaperones, along with antisilencing factor 1, that promotes histone H3-Lys-56 acetylation by the regulation of Ty1 transposition protein 109 (Rtt109) histone acetyltransferase. Here, we report the x-ray crystal structure of Vps75 and carry out biochemical studies to characterize its interaction with Rtt109. We find that the Vps75 structure forms a homodimeric "headphone" architecture that includes an extended helical dimerization domain and earmuff domains at opposite ends and sides of the dimerization domain. Despite the similar overall architecture with the yeast nucleosome assembly protein 1 and human SET/TAF-1beta/INHAT histone chaperones, Vps75 shows several unique features including the relative disposition of the earmuff domains to the dimerization domain, characteristics of the earmuff domains, and a pronounced cleft at the center of the Vps75 dimer. These differences appear to correlate with the unique function of Vps75 to interact with Rtt109 for histone acetylation. Our biochemical studies reveal that two surfaces on the earmuff domain of Vps75 participate in Rtt109 interaction with a stoichiometry of 2:1, thus leaving the pronounced central cleft of the Vps75 dimer largely accessible for histone binding. Taken together, our data provide a structural framework for understanding how Vps75 mediates both nucleosome assembly and histone acetylation by Rtt109.

PubMed: 18723682
DOI: 10.1073/pnas.0802393105

Experimental method

X-RAY DIFFRACTION (2 Å)