3DM7
Crystal Structure of the Vps75 Histone Chaperone
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X6A |
Synchrotron site | NSLS |
Beamline | X6A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-10-13 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9789,0.9791,0.9537 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 79.010, 89.658, 94.069 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.000 |
Rwork | 0.209 |
R-free | 0.24000 |
Structure solution method | MAD |
RMSD bond length | 0.005 |
RMSD bond angle | 1.070 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 45182 | |
<I/σ(I)> | 24.4 | 5.48 |
Completeness [%] | 99.1 | 95.9 |
Redundancy | 13.8 | 11 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.3 | 298 | 25% PEG4000, 100 mM MES, 0.2 M malic acid, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 298K |