3DJW
The thermo- and acido-stable ORF-99 from the archaeal virus AFV1
Summary for 3DJW
| Entry DOI | 10.2210/pdb3djw/pdb |
| Related | 3DF6 |
| Descriptor | ORF99 (1 entity in total) |
| Functional Keywords | archaeal virus, extremophiles, protein fold, lipothrixviridae, viral protein, unknown function |
| Biological source | Acidianus Filamentous Virus 1 |
| Total number of polymer chains | 2 |
| Total formula weight | 23263.96 |
| Authors | Goulet, A.,Spinelli, S.,Prangishvili, D.,van Tilbeurgh, H.,Cambillau, C.,Campanacci, V. (deposition date: 2008-06-24, release date: 2009-06-16, Last modification date: 2023-11-01) |
| Primary citation | Goulet, A.,Spinelli, S.,Blangy, S.,van Tilbeurgh, H.,Leulliot, N.,Basta, T.,Prangishvili, D.,Cambillau, C.,Campanacci, V. The thermo- and acido-stable ORF-99 from the archaeal virus AFV1 Protein Sci., 18:1316-1320, 2009 Cited by PubMed Abstract: Acidianus Filamentous Virus 1 (AFV1), isolated from acidic hot springs, is an enveloped lipid-containing archaeal filamentous virus with a linear double-stranded DNA genome. It infects Acidianus, which is a hyperthermostable archaea growing at 85 degrees C and acidic pHs, below pH 3. AFV1-99, a protein of 99 amino acids of unknown function, has homologues in the archaeal virus families Lipothrixviridae and Rudiviridae. We determined the crystal structure of AFV1-99 at 2.05 A resolution. AFV1-99 has a new fold, is hyperthermostable (up to 95 degrees C) and resists to extreme pH (between pH 0 and 11) and to the combination of high temperature (95 degrees C) and low pH (pH 0). It possesses characteristics of hyperthermostable proteins, such as a high content of charged residues. PubMed: 19472363DOI: 10.1002/pro.122 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (3.1 Å) |
Structure validation
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