3DJW
The thermo- and acido-stable ORF-99 from the archaeal virus AFV1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-08-25 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.934 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 101.411, 101.411, 58.522 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 3.100 |
| R-factor | 0.24976 |
| Rwork | 0.245 |
| R-free | 0.29631 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3DF6 chain A |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.065 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.270 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.101 | 0.556 |
| Number of reflections | 5897 | |
| <I/σ(I)> | 4.4 | 3.3 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 7.5 | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 1.35M Sodium Citrate, 0.1M Sodium Hepes, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
