3CYY

The crystal structure of ZO-1 PDZ2 in complex with the Cx43 peptide

3CYY の概要

• エントリーDOI: 10.2210/pdb3cyy/pdb
• 分子名称: Tight junction protein ZO-1, peptide from Gap junction alpha-1 protein (3 entities in total)
• 機能のキーワード: protein-ligand complex, cell junction, membrane, phosphoprotein, sh3 domain, tight junction, gap junction, transmembrane, peptide binding protein
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Cell membrane; Peripheral membrane protein; Cytoplasmic side: Q07157; Cell membrane; Multi-pass membrane protein: P08050
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 22529.80

構造登録者

Pan, L.,Zhang, M. (登録日: 2008-04-27, 公開日: 2008-09-23, 最終更新日: 2023-11-01)

主引用文献

Chen, J.,Pan, L.,Wei, Z.,Zhao, Y.,Zhang, M.
Domain-swapped dimerization of ZO-1 PDZ2 generates specific and regulatory connexin43-binding sites
Embo J., 27:2113-2123, 2008

PubMed Abstract: PDZ domain scaffold proteins are capable of assembling macromolecular protein complexes in diverse cellular processes through PDZ-mediated binding to a short peptide fragment at the carboxyl tail of target proteins. How each PDZ domain specifically recognizes its target protein(s) remains a major conceptual question, as at least a few out of the several hundred PDZ domains in each eukaryotic genome share overlapping binding properties with any given target protein. Here, we show that the domain-swapped dimerization of zonula occludens-1 PDZ2 generates a distinct interface that functions together with the well-separated canonical carboxyl tail-binding pocket in each PDZ unit in binding to connexin43 (Cx43). We further demonstrate that the charge-charge interaction network formed by residues in the PDZ dimer interface and upstream residues of the Cx43 peptide not only provides the unprecedented interaction specificity for the complex but may also function as a phosphorylation-mediated regulatory switch for the dynamics of the Cx43 gap junctions. Finally, we provide evidence that such domain-swapped dimer assembly also occurs in other PDZ domain scaffold proteins. Therefore, our findings present a new paradigm for understanding how some PDZ domain proteins specifically bind to and regulate the functions of their target proteins.

PubMed: 18636092
DOI: 10.1038/emboj.2008.138

実験手法

X-RAY DIFFRACTION (2.4 Å)