3CF4

Structure of the CODH component of the M. barkeri ACDS complex

Summary for 3CF4

• Entry DOI: 10.2210/pdb3cf4/pdb
• Descriptor: Acetyl-CoA decarboxylase/synthase alpha subunit, Acetyl-CoA decarboxylase/synthase epsilon subunit, IRON/SULFUR CLUSTER, ... (10 entities in total)
• Functional Keywords: methanomicrobia, iron-nikel-sulfur, 4fe-ni-4s, oxidoreductase
• Biological source: Methanosarcina barkeri; More
• Total number of polymer chains: 2
• Total formula weight: 110019.32

Authors

Gong, W.,Hao, B.,Wei, Z.,Ferguson Jr., D.J.,Tallant, T.,Krzycki, J.A.,Chan, M.K. (deposition date: 2008-03-01, release date: 2008-07-22, Last modification date: 2024-02-21)

Primary citation

Gong, W.,Hao, B.,Wei, Z.,Ferguson Jr., D.J.,Tallant, T.,Krzycki, J.A.,Chan, M.K.
Structure of the alpha2 epsilon2 Ni-dependent CO dehydrogenase component of the Methanosarcina barkeri acetyl-CoA decarboxylase/synthase complex
Proc.Natl.Acad.Sci.USA, 105:9558-9563, 2008

PubMed Abstract: Ni-dependent carbon monoxide dehydrogenases (Ni-CODHs) are a diverse family of enzymes that catalyze reversible CO:CO(2) oxidoreductase activity in acetogens, methanogens, and some CO-using bacteria. Crystallography of Ni-CODHs from CO-using bacteria and acetogens has revealed the overall fold of the Ni-CODH core and has suggested structures for the C cluster that mediates CO:CO(2) interconversion. Despite these advances, the mechanism of CO oxidation has remained elusive. Herein, we report the structure of a distinct class of Ni-CODH from methanogenic archaea: the alpha(2)epsilon(2) component from the alpha(8)beta(8)gamma(8)delta(8)epsilon(8) CODH/acetyl-CoA decarbonylase/synthase complex, an enzyme responsible for the majority of biogenic methane production on Earth. The structure of this Ni-CODH component provides support for a hitherto unobserved state in which both CO and H(2)O/OH(-) bind to the Ni and the exogenous FCII iron of the C cluster, respectively, and offers insight into the structures and functional roles of the epsilon-subunit and FeS domain not present in nonmethanogenic Ni-CODHs.

PubMed: 18621675
DOI: 10.1073/pnas.0800415105

Experimental method

X-RAY DIFFRACTION (2 Å)