3CDU

Crystal structure of coxsackievirus B3 RNA-dependent RNA polymerase (3Dpol) in complex with a pyrophosphate

3CDU の概要

• エントリーDOI: 10.2210/pdb3cdu/pdb
• 関連するPDBエントリー: 1ILZ 1RA6 1XR5 2E9Z 2EC0 3CDW
• 分子名称: RNA-directed RNA polymerase 3D-POL, CHLORIDE ION, SULFATE ION, ... (7 entities in total)
• 機能のキーワード: coxsackievirus, rna-dependent rna polymerase, vizier viral enzymes involved in replication, transferase
• 由来する生物種: Coxsackievirus B3
• 細胞内の位置: Protein VP2: Virion. Protein VP3: Virion. Protein VP1: Virion. Protein 2B: Host cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side (Potential). Protein 2C: Host cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side (Potential). Protein 3A: Host cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side (Potential). Protein 3B: Virion (Potential). Picornain 3C: Host cytoplasm (Potential). RNA-directed RNA polymerase 3D-POL: Host cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side (Potential): P03313
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 56879.69

構造登録者

Gruez, A.,Selisko, B.,Roberts, M.,Bricogne, G.,Bussetta, C.,Canard, B. (登録日: 2008-02-27, 公開日: 2008-07-22, 最終更新日: 2023-11-01)

主引用文献

Gruez, A.,Selisko, B.,Roberts, M.,Bricogne, G.,Bussetta, C.,Jabafi, I.,Coutard, B.,De Palma, A.M.,Neyts, J.,Canard, B.
The crystal structure of coxsackievirus B3 RNA-dependent RNA polymerase in complex with its protein primer VPg confirms the existence of a second VPg binding site on Picornaviridae polymerases
J.Virol., 82:9577-9590, 2008

PubMed Abstract: The RNA-dependent RNA polymerase (RdRp) is a central piece in the replication machinery of RNA viruses. In picornaviruses this essential RdRp activity also uridylates the VPg peptide, which then serves as a primer for RNA synthesis. Previous genetic, binding, and biochemical data have identified a VPg binding site on poliovirus RdRp and have shown that is was implicated in VPg uridylation. More recent structural studies have identified a topologically distinct site on the closely related foot-and-mouth disease virus RdRp supposed to be the actual VPg-primer-binding site. Here, we report the crystal structure at 2.5-A resolution of active coxsackievirus B3 RdRp (also named 3D(pol)) in a complex with VPg and a pyrophosphate. The pyrophosphate is situated in the active-site cavity, occupying a putative binding site either for the coproduct of the reaction or an incoming NTP. VPg is bound at the base of the thumb subdomain, providing first structural evidence for the VPg binding site previously identified by genetic and biochemical methods. The binding mode of VPg to CVB3 3D(pol) at this site excludes its uridylation by the carrier 3D(pol). We suggest that VPg at this position is either uridylated by another 3D(pol) molecule or that it plays a stabilizing role within the uridylation complex. The CVB3 3D(pol)/VPg complex structure is expected to contribute to the understanding of the multicomponent VPg-uridylation complex essential for the initiation of genome replication of picornaviruses.

PubMed: 18632861
DOI: 10.1128/JVI.00631-08

実験手法

X-RAY DIFFRACTION (2.1 Å)