3C25

Crystal Structure of NotI Restriction Endonuclease Bound to Cognate DNA

Replaces:  3BVR

Summary for 3C25

• Entry DOI: 10.2210/pdb3c25/pdb
• Related: 3BVQ
• Descriptor: DNA (5'-D(*DCP*DGP*DGP*DAP*DGP*DGP*DCP*DGP*DCP*DGP*DGP*DCP*DCP*DGP*DCP*DGP*DCP*DCP*DGP*DCP*DCP*DG)-3'), DNA (5'-D(*DCP*DGP*DGP*DCP*DGP*DGP*DCP*DGP*DCP*DGP*DGP*DCP*DCP*DGP*DCP*DGP*DCP*DCP*DTP*DCP*DCP*DG)-3'), NotI restriction endonuclease, ... (6 entities in total)
• Functional Keywords: protein-dna complex, restriction enzyme fold, pd-(d/e)-xk, restriction endonuclease, rare-cutting, fe-cys4 motif, iron-sulfur protein, hydrolase-dna complex, hydrolase/dna
• Biological source: Nocardia otitidiscaviarum; More
• Total number of polymer chains: 4
• Total formula weight: 98729.41

Authors

Lambert, A.R.,Sussman, D.,Shen, B.,Stoddard, B.L. (deposition date: 2008-01-24, release date: 2008-02-12, Last modification date: 2024-02-21)

• Primary citation: Lambert, A.R.,Sussman, D.,Shen, B.,Maunus, R.,Nix, J.,Samuelson, J.,Xu, S.Y.,Stoddard, B.L.; Structures of the Rare-Cutting Restriction Endonuclease NotI Reveal a Unique Metal Binding Fold Involved in DNA Binding.; Structure, 16:558-569, 2008; PubMed: 18400177; DOI: 10.1016/j.str.2008.01.017

Experimental method

X-RAY DIFFRACTION (2.5 Å)