3C06

Lactobacillus CASEI Thymidylate Synthase Ternary Complex With DUMP and the Phtalimidic Derivative 14C in Multiple Binding Modes-Mode 1

3C06 の概要

• エントリーDOI: 10.2210/pdb3c06/pdb
• 関連するPDBエントリー: 1TLS 3BNZ 3BYX 3BZ0 3C0A
• 分子名称: Thymidylate synthase, 2'-DEOXYURIDINE 5'-MONOPHOSPHATE, 2-(2-chloropyridin-4-yl)-4-methyl-1H-isoindole-1,3(2H)-dione, ... (4 entities in total)
• 機能のキーワード: methyltransferase, structure-based drug design, nucleotide biosynthesis, transferase
• 由来する生物種: Lactobacillus casei
• 細胞内の位置: Cytoplasm: P00469
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 37211.32

構造登録者

Leone, R.,Mangani, S.,Cancian, L.,Costi, M.P.,Luciani, R.,Ferrari, S. (登録日: 2008-01-18, 公開日: 2009-01-20, 最終更新日: 2023-11-01)

主引用文献

Mangani, S.,Cancian, L.,Leone, R.,Pozzi, C.,Lazzari, S.,Luciani, R.,Ferrari, S.,Costi, M.P.
Identification of the binding modes of N-phenylphthalimides inhibiting bacterial thymidylate synthase through X-ray crystallography screening
J.Med.Chem., 54:5454-5467, 2011

PubMed Abstract: To identify specific bacterial thymidylate synthase (TS) inhibitors, we exploited phenolphthalein (PTH), which inhibits both bacterial and human enzymes. The X-ray crystal structure of Lactobacillus casei TS (LcTS) that binds PTH showed multiple binding modes of the inhibitor, which prevented a classical structure-based drug design approach. To overcome this issue, we synthesized two phthalimidic libraries that were tested against TS enzymes and then we performed X-ray crystallographic screening of the active compounds. Compounds 6A, 8A, and 12A showed 40-fold higher affinity for bacterial TS than human TS. The X-ray crystallographic screening characterized the binding mode of six inhibitors in complexes with LcTS. Of these, 20A, 23A, and 24A showed a common unique binding mode, whereas 8A showed a different, unique binding mode. A comparative analysis of the LcTS X-ray complexes that were obtained with the pathogenic TS enabled the selection of compounds 8A and 23A as specific compounds and starting points to be exploited for the specific inhibition of pathogen enzymes.

PubMed: 21696158
DOI: 10.1021/jm2005018

実験手法

X-RAY DIFFRACTION (2.6 Å)