3BTS

Crystal structure of a ternary complex of the transcriptional repressor Gal80p (Gal80S0 [G301R]) and the acidic activation domain of Gal4p (aa 854-874) from Saccharomyces cerevisiae with NAD

Summary for 3BTS

• Entry DOI: 10.2210/pdb3bts/pdb
• Related: 3BTU 3BTV
• Descriptor: Galactose/lactose metabolism regulatory protein GAL80, Regulatory protein GAL4, NICOTINAMIDE-ADENINE-DINUCLEOTIDE, ... (4 entities in total)
• Functional Keywords: eukaryotic transcription complex, nad, rossmann fold, acetylation, carbohydrate metabolism, dna-binding, galactose metabolism, repressor, transcription regulation, activator, metal-binding, nucleus, phosphoprotein, zinc, transcription
• Biological source: Saccharomyces cerevisiae (baker's yeast); More
• Cellular location: Nucleus: P04386
• Total number of polymer chains: 4
• Total formula weight: 103850.95

Authors

Kumar, P.R.,Joshua-Tor, L. (deposition date: 2007-12-30, release date: 2008-03-04, Last modification date: 2023-08-30)

Primary citation

Kumar, P.R.,Yu, Y.,Sternglanz, R.,Johnston, S.A.,Joshua-Tor, L.
NADP regulates the yeast GAL induction system.
Science, 319:1090-1092, 2008

PubMed Abstract: Transcriptional regulation of the galactose-metabolizing genes in Saccharomyces cerevisiae depends on three core proteins: Gal4p, the transcriptional activator that binds to upstream activating DNA sequences (UAS(GAL)); Gal80p, a repressor that binds to the carboxyl terminus of Gal4p and inhibits transcription; and Gal3p, a cytoplasmic transducer that, upon binding galactose and adenosine 5'-triphosphate, relieves Gal80p repression. The current model of induction relies on Gal3p sequestering Gal80p in the cytoplasm. However, the rapid induction of this system implies that there is a missing factor. Our structure of Gal80p in complex with a peptide from the carboxyl-terminal activation domain of Gal4p reveals the existence of a dinucleotide that mediates the interaction between the two. Biochemical and in vivo experiments suggests that nicotinamide adenine dinucleotide phosphate (NADP) plays a key role in the initial induction event.

PubMed: 18292341
DOI: 10.1126/science.1151903

Experimental method

X-RAY DIFFRACTION (2.7 Å)