3BM3

Restriction endonuclease PspGI-substrate DNA complex

Summary for 3BM3

• Entry DOI: 10.2210/pdb3bm3/pdb
• Descriptor: DNA (5'-D(*CP*AP*TP*CP*CP*AP*GP*GP*TP*AP*C)-3'), DNA (5'-D(*GP*GP*TP*AP*CP*CP*TP*GP*GP*AP*T)-3'), PspGI restriction endonuclease, ... (5 entities in total)
• Functional Keywords: endonuclease-dna complex, restriction enzyme, pspgi, base flipping, hydrolase-dna complex, hydrolase/dna
• Biological source: Pyrococcus sp. GI-H
• Total number of polymer chains: 4
• Total formula weight: 71330.09

Authors

Szczepanowski, R.H.,Carpenter, M.,Czapinska, H.,Tamulaitis, G.,Siksnys, V.,Bhagwat, A.,Bochtler, M. (deposition date: 2007-12-12, release date: 2008-09-16, Last modification date: 2021-10-20)

• Primary citation: Szczepanowski, R.H.,Carpenter, M.,Czapinska, H.,Tamulaitis, G.,Siksnys, V.,Bhagwat, A.,Bochtler, M.; A direct crystallographic demonstration that Type II restriction endonuclease PspGI flips nucleotides; To be Published,

Experimental method

X-RAY DIFFRACTION (1.7 Å)