3BM3
Restriction endonuclease PspGI-substrate DNA complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (C) | DNA (5'-D(*CP*AP*TP*CP*CP*AP*GP*GP*TP*AP*C)-3') | polymer | 11 | 3318.2 | 1 | |||
| 2 | B (D) | DNA (5'-D(*GP*GP*TP*AP*CP*CP*TP*GP*GP*AP*T)-3') | polymer | 11 | 3389.2 | 1 | |||
| 3 | C, D (A, B) | PspGI restriction endonuclease | polymer | 272 | 32215.3 | 2 | UniProt (O93646) Pfam (PF09019) | Pyrococcus sp. GI-H | |
| 4 | E (B) | CITRIC ACID | non-polymer | 192.1 | 1 | Chemie (CIT) | |||
| 5 | F, G, H, I (C, D, A, B) | water | water | 18.0 | 293 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 272 (UniProt: O93646)
| PDB | External Database | Details |
|---|---|---|
| Ala 138 | Asp 138 | engineered mutation |
| Thr 146 | Ala 146 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 71138.0 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 192.1 | |
| All* | Total formula weight | 71330.1 |
