3ANW

A protein complex essential initiation of DNA replication

Summary for 3ANW

• Entry DOI: 10.2210/pdb3anw/pdb
• Descriptor: Putative uncharacterized protein (3 entities in total)
• Functional Keywords: sld5 superfamily, dna replication, replication
• Biological source: Thermococcus kodakarensis (Thermococcus kodakaraensis); More
• Total number of polymer chains: 2
• Total formula weight: 41653.08

Authors

Oyama, T.,Shirai, T.,Nagasawa, N.,Ishino, Y.,Morikawa, K. (deposition date: 2010-09-10, release date: 2011-07-27, Last modification date: 2024-03-13)

Primary citation

Oyama, T.,Ishino, S.,Fujino, S.,Ogino, H.,Shirai, T.,Mayanagi, K.,Saito, M.,Nagasawa, N.,Ishino, Y.,Morikawa, K.
Architectures of archaeal GINS complexes, essential DNA replication initiation factors
Bmc Biol., 9:28-28, 2011

PubMed Abstract: In the early stage of eukaryotic DNA replication, the template DNA is unwound by the MCM helicase, which is activated by forming a complex with the Cdc45 and GINS proteins. The eukaryotic GINS forms a heterotetramer, comprising four types of subunits. On the other hand, the archaeal GINS appears to be either a tetramer formed by two types of subunits in a 2:2 ratio (α2β2) or a homotetramer of a single subunit (α4). Due to the low sequence similarity between the archaeal and eukaryotic GINS subunits, the atomic structures of the archaeal GINS complexes are attracting interest for comparisons of their subunit architectures and organization.

PubMed: 21527023
DOI: 10.1186/1741-7007-9-28

Experimental method

X-RAY DIFFRACTION (2.65 Å)