3ANW
A protein complex essential initiation of DNA replication
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-07-05 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 76.343, 76.343, 181.333 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.350 - 2.650 |
R-factor | 0.262 |
Rwork | 0.262 |
R-free | 0.31000 |
Structure solution method | MIR |
RMSD bond length | 0.010 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHARP |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.350 | 2.740 |
High resolution limit [Å] | 2.650 | 2.650 |
Rmerge | 0.043 | 0.388 |
Number of reflections | 16278 | |
<I/σ(I)> | 17.6 | 9.9 |
Completeness [%] | 99.4 | 100 |
Redundancy | 14 | 14 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1M HEPES, 0.2M magnesium chloride, 24% PEG 400, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |