3AMZ

Bovine Xanthine Oxidoreductase urate bound form

Summary for 3AMZ

Entry DOI	10.2210/pdb3amz/pdb
Related	3AM9 3AN1
Descriptor	Xanthine dehydrogenase/oxidase, URIC ACID, FE2/S2 (INORGANIC) CLUSTER, ... (11 entities in total)
Functional Keywords	reaction intermediate, oxidoreductase
Biological source	Bos taurus (Bovine)
Cellular location	Cytoplasm (By similarity): P80457
Total number of polymer chains	2
Total formula weight	299518.96
Authors	Okamoto, K.,Eger, B.T.,Pai, E.F.,Nishino, T. (deposition date: 2010-08-27, release date: 2010-12-01, Last modification date: 2023-11-01)
Primary citation	Okamoto, K.,Kawaguchi, Y.,Eger, B.T.,Pai, E.F.,Nishino, T. Crystal Structures of Urate Bound Form of Xanthine Oxidoreductase: Substrate Orientation and Structure of the Key Reaction Intermediate J.Am.Chem.Soc., 132:17080-17083, 2010 Cited by PubMed Abstract: Two contradictory models have been proposed for the binding mode of the substrate xanthine to and its activation mechanism by xanthine oxidoreductase. In an effort to distinguish between the two models, we determined the crystal structures of the urate complexes of the demolybdo-form of the D428A mutant of rat xanthine oxidoreductase at 1.7 Å and of the reduced bovine milk enzyme at 2.1 Å, the latter representing a reaction intermediate. The results clearly indicate the catalytically relevant binding mode of the substrate xanthine. PubMed: 21077683 DOI: 10.1021/ja1077574 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.1 Å)

Structure validation