Structure of Gankyrin-S6ATPase photo-cross-linked site-specifically, and incoporated by genetic code expansion

Summary for 3AJI

Descriptor26S proteasome non-ATPase regulatory subunit 10, Proteasome (Prosome, macropain) 26S subunit, ATPase, 4 (3 entities in total)
Functional Keywordsgankyrin, s6 atpase, p-benzoyl-l-phenylalanine, pbpa, amber suppression, structural genomics, riken structural genomics/proteomics initiative, rsgi, chaperone-protein binding complex, chaperone/protein binding
Biological sourceMus musculus (mouse)
Cellular locationCytoplasm (By similarity) Q9Z2X2
Total number of polymer chains4
Total molecular weight69949.58
Sato, S.,Mimasu, S.,Sato, A.,Hino, N.,Sakamoto, K.,Umehara, T.,Yokoyama, S.,RIKEN Structural Genomics/Proteomics Initiative (RSGI) (deposition date: 2010-06-07, release date: 2010-12-22, Last modification date: 2014-01-29)
Primary citation
Sato, S.,Mimasu, S.,Sato, A.,Hino, N.,Sakamoto, K.,Umehara, T.,Yokoyama, S.
Crystallographic study of a site-specifically cross-linked protein complex with a genetically incorporated photoreactive amino acid
Biochemistry, 50:250-257, 2011
PubMed: 21128684 (PDB entries with the same primary citation)
DOI: 10.1021/bi1016183
MImport into Mendeley
Experimental method

Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers 0.224190 8.7% 1.8%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution
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PDB entries from 2020-12-02