3AJI
Structure of Gankyrin-S6ATPase photo-cross-linked site-specifically, and incoporated by genetic code expansion
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-12-15 |
Detector | RAYONIX MX225HE |
Spacegroup name | H 3 |
Unit cell lengths | 103.164, 103.164, 154.993 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.930 - 2.050 |
R-factor | 0.171 |
Rwork | 0.171 |
R-free | 0.22900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2dvw |
RMSD bond length | 0.032 |
RMSD bond angle | 2.800 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.180 |
High resolution limit [Å] | 2.050 | 2.050 |
Number of reflections | 38604 | |
<I/σ(I)> | 20.7 | 3.2 |
Completeness [%] | 100.0 | 99.9 |
Redundancy | 4.2 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 31% polyethylene glycol 4000, 0.26M MgCl2, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 31% polyethylene glycol 4000, 0.26M MgCl2, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |