3SIT

Crystal structure of porcine CRW-8 Rotavirus VP8* in complex with aceramido-GM3

Summary for 3SIT

• Entry DOI: 10.2210/pdb3sit/pdb
• Related: 2I2S 3SIS
• Related PRD ID: PRD_900025
• Descriptor: Outer capsid protein VP4, N-acetyl-alpha-neuraminic acid-(2-3)-beta-D-galactopyranose-(1-4)-alpha-D-glucopyranose, SODIUM ION, ... (5 entities in total)
• Functional Keywords: beta sandwich, lectin, gm3, sugar binding protein, viral protein
• Biological source: Porcine rotavirus (RV-A)
• Cellular location: Outer capsid protein VP4: Virion. Outer capsid protein VP8*: Virion. Outer capsid protein VP5*: Virion: P0C6Y8
• Total number of polymer chains: 2
• Total formula weight: 38468.46

Authors

Blanchard, H.,Yu, X. (deposition date: 2011-06-20, release date: 2011-09-28, Last modification date: 2023-09-13)

Primary citation

Yu, X.,Coulson, B.S.,Fleming, F.E.,Dyason, J.C.,von Itzstein, M.,Blanchard, H.
Novel structural insights into rotavirus recognition of ganglioside glycan receptors.
J.Mol.Biol., 413:929-939, 2011

PubMed Abstract: Rotaviruses ubiquitously infect children under the age of 5, being responsible for more than half a million diarrhoeal deaths each year worldwide. Host cell oligosaccharides containing sialic acid(s) are critical for attachment by rotaviruses. However, to date, no detailed three-dimensional atomic model showing the exact rotavirus interactions with these glycoconjugate receptors has been reported. Here, we present the first crystallographic structures of the rotavirus carbohydrate-recognizing protein VP8* in complex with ganglioside G(M3) glycans. In combination with assessment of the inhibition of rotavirus infectivity by N-acetyl and N-glycolyl forms of this ganglioside, our results reveal key details of rotavirus-ganglioside G(M3) glycan recognition. In addition, they show a direct correlation between the carbohydrate specificities exhibited by VP8* from porcine and by monkey rotaviruses and the respective infectious virus particles. These novel results also indicate the potential binding interactions of rotavirus VP8* with other sialic acid-containing gangliosides.

PubMed: 21945555
DOI: 10.1016/j.jmb.2011.09.005

Experimental method

X-RAY DIFFRACTION (1.8 Å)