3P8N

Crystal structure of HCV NS3/NS4A protease complexed with BI 201335

Summary for 3P8N

• Entry DOI: 10.2210/pdb3p8n/pdb
• Related: 3P8O
• Related PRD ID: PRD_000845
• Descriptor: HCV serine protease NS3, HCV non-structural protein 4A, SODIUM ION, ... (5 entities in total)
• Functional Keywords: hepatitis c virus, ns3, ns4a, halogen bond, serine protease, hydrolase-hydrolase inhibitor complex, hydrolase/hydrolase inhibitor
• Biological source: Hepatitis C virus (HCV)
• Cellular location: Core protein p21: Host endoplasmic reticulum membrane ; Single-pass membrane protein . Core protein p19: Virion . Envelope glycoprotein E1: Virion membrane ; Single-pass type I membrane protein . Envelope glycoprotein E2: Virion membrane ; Single-pass type I membrane protein . p7: Host endoplasmic reticulum membrane ; Multi-pass membrane protein . Protease NS2-3: Host endoplasmic reticulum membrane ; Multi-pass membrane protein . Serine protease NS3: Host endoplasmic reticulum membrane ; Peripheral membrane protein . Non-structural protein 4A: Host endoplasmic reticulum membrane ; Single-pass type I membrane protein . Non-structural protein 4B: Host endoplasmic reticulum membrane ; Multi-pass membrane protein . Non-structural protein 5A: Host endoplasmic reticulum membrane; Peripheral membrane protein. RNA-directed RNA polymerase: Host endoplasmic reticulum membrane ; Single-pass type I membrane protein : P26662
• Total number of polymer chains: 4
• Total formula weight: 44743.45

Authors

Lemke, C.T. (deposition date: 2010-10-14, release date: 2011-01-26, Last modification date: 2017-11-08)

• Primary citation: Lemke, C.T.,Goudreau, N.,Zhao, S.,Hucke, O.,Thibeault, D.,Llinas-Brunet, M.,White, P.W.; Combined X-ray, NMR, and kinetic analyses reveal uncommon binding characteristics of the hepatitis C virus NS3-NS4A protease inhibitor BI 201335.; J.Biol.Chem., 286:11434-11443, 2011; PubMed: 21270126; DOI: 10.1074/jbc.M110.211417

Experimental method

X-RAY DIFFRACTION (1.9 Å)