3KUY

DNA Stretching in the Nucleosome Facilitates Alkylation by an Intercalating Antitumor Agent

Summary for 3KUY

• Entry DOI: 10.2210/pdb3kuy/pdb
• Related: 2NZD
• Descriptor: Histone H3.2, Histone H4, Histone H2A, ... (8 entities in total)
• Functional Keywords: nucleosome, chromatin, dna stretching, intercalation, alkylation, chromosomal protein, dna-binding, methylation, nucleosome core, nucleus, structural protein-dna complex, structural protein/dna
• Biological source: Xenopus laevis (African clawed frog); More
• Cellular location: Nucleus: Q6AZJ8 Q92130; Nucleus (By similarity): 3KUY 3KUY
• Total number of polymer chains: 10
• Total formula weight: 196764.46

Authors

Wu, B.,Davey, C.A. (deposition date: 2009-11-28, release date: 2010-01-05, Last modification date: 2023-11-01)

Primary citation

Davey, G.E.,Wu, B.,Dong, Y.,Surana, U.,Davey, C.A.
DNA stretching in the nucleosome facilitates alkylation by an intercalating antitumour agent
Nucleic Acids Res., 38:2081-2088, 2010

PubMed Abstract: DNA stretching in the nucleosome core can cause dramatic structural distortions, which may influence compaction and factor recognition in chromatin. We find that the base pair unstacking arising from stretching-induced extreme minor groove kinking near the nucleosome centre creates a hot spot for intercalation and alkylation by a novel anticancer compound. This may have far reaching implications for how chromatin structure can influence binding of intercalator species and indicates potential for the development of site selective DNA-binding agents that target unique conformational features of the nucleosome.

PubMed: 20026584
DOI: 10.1093/nar/gkp1174

Experimental method

X-RAY DIFFRACTION (2.9 Å)