3K9P
The crystal structure of E2-25K and ubiquitin complex
Summary for 3K9P
| Entry DOI | 10.2210/pdb3k9p/pdb |
| Related | 3K9O |
| Descriptor | Ubiquitin-conjugating enzyme E2 K, Ubiquitin (2 entities in total) |
| Functional Keywords | e2-25k, ubiquitin, complex structure, atp-binding, isopeptide bond, ligase, nucleotide-binding, ubl conjugation pathway, nucleus, phosphoprotein, ligase-signaling protein complex, ligase/signaling protein |
| Biological source | Homo sapiens (human) More |
| Cellular location | Cytoplasm (By similarity): P61086 |
| Total number of polymer chains | 2 |
| Total formula weight | 32953.57 |
| Authors | Kang, G.B.,Ko, S.,Song, S.M.,Lee, W.,Eom, S.H. (deposition date: 2009-10-16, release date: 2010-09-08, Last modification date: 2024-03-20) |
| Primary citation | Ko, S.,Kang, G.B.,Song, S.M.,Lee, J.-G.,Shin, D.Y.,Yun, J.-H.,Sheng, Y.,Cheong, C.,Jeon, Y.H.,Jung, Y.-K.,Arrowsmith, C.H.,Avvakumov, G.V.,Dhe-Paganon, S.,Yoo, Y.J.,Eom, S.H.,Lee, W. Structural basis of E2-25K/UBB+1 interaction leading to proteasome inhibition and neurotoxicity J.Biol.Chem., 285:36070-36080, 2010 Cited by PubMed Abstract: E2-25K/Hip2 is an unusual ubiquitin-conjugating enzyme that interacts with the frameshift mutant of ubiquitin B (UBB(+1)) and has been identified as a crucial factor regulating amyloid-β neurotoxicity. To study the structural basis of the neurotoxicity mediated by the E2-25K-UBB(+1) interaction, we determined the three-dimensional structures of UBB(+1), E2-25K and the E2-25K/ubiquitin, and E2-25K/UBB(+1) complex. The structures revealed that ubiquitin or UBB(+1) is bound to E2-25K via the enzyme MGF motif and residues in α9 of the enzyme. Polyubiquitylation assays together with analyses of various E2-25K mutants showed that disrupting UBB(+1) binding markedly diminishes synthesis of neurotoxic UBB(+1)-anchored polyubiquitin. These results suggest that the interaction between E2-25K and UBB(+1) is critical for the synthesis and accumulation of UBB(+1)-anchored polyubiquitin, which results in proteasomal inhibition and neuronal cell death. PubMed: 20826778DOI: 10.1074/jbc.M110.145219 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.8 Å) |
Structure validation
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